国際心臓血管研究ジャーナル

The Relationship of HSP-70 with Calcineurin, Sod and Catalase Post-Acute Myocardial Infarction in Wistar Rats Model

Johanes Nugroho1,2*, Christo Darius2, Maria Yolanda Probohoesodo3, Suhartono Taat Putra4 and Cornelia Ghea5

Background: HSP-70s help to reduce infarct area through unclear mechanism. It appears that HSP-70 activates calcineurin and induce antioxidant enzymes such as Superoxide Dismutase (SOD) and catalase. Therefore, we sought to investigate the relationship of HSP-70 with calcineurin, SOD and catalase post-Acute Myocardial Infarction (AMI). Methods: An experimental study involved 24 Wistar rats as models of chronic coronary occlusion. The rats were randomly divided into 4 groups: no-intervention after AMI (N), sedentary intervention after AMI (S), exercise intervention after AMI (E), and sham (C). Intervention consisted of 2 weeks of recovery then 4 weeks of sedentary for group S or exercise for group E. HSP-70, calcineurin, SOD, and catalase expression in heart were evaluated the difference among groups. Correlation between HSP-70 to other proteins was analysed also. Results: HSP-70 and calcineurin was higher in group S and E compared to group N and C. HSP-70 (MD=0.97, 95% CI 0.60 to 1.34), calcineurin (MD=1.25, 95% CI 0.68 to 1.82, p<0.05), catalase (MD=0.57, 95% CI 0.25 to 0.88, p<0.05), and SOD (0.42, 95% CI 0.14 to 0.69, p<0.05) significantly higher in Group E compared to S. Sham group had higher SOD and catalase activity than group E. HSP-70 was correlated with calcineurin (r=0.856, p<0.05). HSP-70 was correlated with catalase and SOD when the analysis included only groups who had intervention. Conclusion: HSP-70 correlated with calcineurin and stimulated an increase in calcineurin activity.