細胞生物学: 研究と治療

Hemostasis property of Malian herbal plant used to manage bleeding event

Diallo Yacouba L  

Introduction: Bleeding diseases management is a big challenge in developing countries where diagnosis and drug access are not easy. In Mali 80-90% of the population frequently used medicinal plant with a good response. Unfortunately, knowledge on these plants is undocumented. Here, we investigated ten herbal plants currently used by traditional practitioners in Dioila district (Mali) to treat bleeding conditions.  e aim of this study was to investigate the coagulation properties of these plants and identi­ ed the substance responsible for di erent hemostasis properties. Materials & Methods:  e hemostasis properties of water, ethanol and dichloromethane extracts from ten plants have been investigated.  e plants were selected a er ethnobotanical survey conducted in Dioila area in Mali. Fi een traditional practitioners were interviewed in the survey and the ten plants currently used according to their high level of ­ delity were retained for this study.  e e ect of the extracts on hemostasis parameters was investigated using whole blood from healthy donor.Acarbose is a drug to treat type-2 diabetes mellitus and obesity by inhibiting α-glucosidase that releases glucose from higher carbohydrates, and therefore its detection is of particular signi­ cance from the diagnostic viewpoint.  e saccharide sensing results of further interesting approach by an in situ hybrid sensor with Cur and PyPT in Figure 1b and their detailed supramolecular complexation will be discuss All extracts were incubated with whole blood at the ­ nal concentration of 0.25 g/L. Activating platelet time aPTT and thrombin time were measured using coagulation automate (STA satellite®) at 0 and 30 min a er incubation. Bu er was used as a control in the same condition. Results were expressed as ratio for aPTT and percentage for  rombin time. All tests were performed in double. Results: We have investigated the e ect of twelve extracts from ten plants on aPTT and thrombin time at (0 and 30 min) a er incubation. aPTT measurement directly a er incubation showed that eleven extracts gave a result lower than 1.2. Only extracts from Pteleopsis myrtifolia bark and trunk, induced an aPTT beyond 1.2. A er 30 min incubation, aPTT value from all extracts was lower than 1.2. In contrast, it seems that prothrombin time was not strongly modi­ ed by any extract. Conclusion: Some extracts from herbal plants modi­ ed aPPT which could be associated to a hemostatic e ect. More investigations are needed in order to con­ rm these ­ ndings. ed.